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Vector Laboratories vectashield hardset mounting media with dapi
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Vectashield Hardset Mounting Media With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotium everbrite hardset mounting media
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Everbrite Hardset Mounting Media, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vectashield hardset antifade mounting media
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Vectashield Hardset Antifade Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vectashield
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Vectashield, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories dapi
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories 4 6 diamidino 2 phenylindole
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
4 6 Diamidino 2 Phenylindole, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotium media
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Media, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vectashield hard set mounting media
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Vectashield Hard Set Mounting Media, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Laboratories vectashield hardset mounting media dapi counterstain
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Vectashield Hardset Mounting Media Dapi Counterstain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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98
Vector Laboratories diamidino 2 phenylindole
Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and <t>DAPI</t> (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.
Diamidino 2 Phenylindole, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and DAPI (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.

Journal: Journal of cell science

Article Title: Loss of Ca 2+ entry via Orai-TRPC1 induces ER stress, initiating immune activation in macrophages.

doi: 10.1242/jcs.237610

Figure Lengend Snippet: Fig. 5. Tunicamycin, brefeldin A and SKF96365 treatment induce autophagy and apoptosis in Raw 264.7 macrophages. (A) Representative immunoblot images showing expression of markers for the induction of autophagy and apoptosis in Raw 264.7 macrophages treated with 10 µM Tuni, BFA or SKF for 12 h. (B) Quantification of densitometric values (mean±s.d.) from bands as shown in A. (C) Representative microscopy images of Raw 264.7 macrophages stained for LC3B (green), CHOP (red) and DAPI (blue) after cells were exposed to 10 µM Tuni, BFA or SKF for 12 h. **P<0.01; ***P<0.001 by one-way ANOVA test. The data shown are representative of three independent experiments. Scale bars: 20 µm.

Article Snippet: Thereafter, the slides were washed again, and coverslip mounted using VECTASHIELD HardSet Mounting Media with DAPI (Vector Laboratories, Inc.).

Techniques: Western Blot, Expressing, Microscopy, Staining